Chemical Science & Engineering Research
Title
Development and Validation of UV Spectroscopic First Derivative Method for Simultaneous Estimation of Aceclofenac and Serratiopeptidase in Synthetic Mixture
Authors
Om K. Hudka,*a Brijesh R. Erda,a Khushali J. Chothania and Bhavik R. Janib
aSchool of Science, R.K University, Rajkot , Gujarat , India.
bSchool of Pharmacy, R.K University , Rajkot , Gujarat , India.
*Corresponding author E-mail address: omhudka2002@gmail.com (Om K. Hudka)
Article History
Publication details: Received: 02nd April 2022; Revised: 11th May 2022; Accepted: 11th May 2022; Published: 24th May 2022
Cite this article
Hudka Om.K.; Erda B.R.; Chothani K.J.; Jani B.R. Development and Validation of UV Spectroscopic First Derivative Method for Simultaneous Estimation of Aceclofenac and Serratiopeptidase in Synthetic Mixture. Chem. Sci. Eng. Res., 2022, 4(10), 5-8.
Abstract
A UV- visible Spectrophotometer method has been developed and validation for Simultaneous estimation of Aceclofenac and Serratiopeptidase in tablet dosage form using double form using double beam UV Spectrophotometer of thermo Electron Corporation with (Methanol + Water) as a solvent. Absorption maxima of Aceclofenac in Methanol and Serratiopeptidase in Methanol diluted with water was found to be 274 nm and 279 nm, respectively. Beer’s law was obeyed in the concentration range 10-50 µg/ml for Aceclofenac and 1.5-7.5 µg/ml Serratiopeptidase. The methods allow rapid analysis of binary pharmaceutical formulation with accuracy, precision. The method was found to be simple, accurate, and precise.
Keywords
Aceclofenac; Simultaneous Estimation; Q-analysis; Serratiopeptidase; Isoabsorptive Point
