Articles - Chemical Science & Engineering Research

Abstract

Denture cleansing is essential to prevent cross contamination. This study aimed to investigate the effect of different disinfecting agents on biofilm and surface roughness of denture base material. Heat cured acrylic resin was used to prepare the tested samples. The disinfectants used are sodium hypochlorite 5% (SHC) - chlorhexidine gluconate 0.12% (CHX) and hydrogen peroxide 6% (HPO). A total of seventy-four discs’ shape was prepared. The microorganisms isolated by a swab from the oral cavity and allowed to grow in their selected culture. The samples were divided into three main groups according to disinfectant agents. All samples were placed in the incubator at 37^oC for 24h to allow growth of the microorganism. All groups were disinfected by immersion in disinfectant for 10 minutes. The surface roughness (Ra-μm) was analyzed with a surface roughness profilometer. SHC groups showed no signs of microorganism growth (St. viridans - S. aureus - C. albicans). HPO showed growth of St. viridans while no signs of C. albicans and S. aureus growth. The CHX showed no signs of microorganism growth (St. viridans - S. aureus - C. albicans). Regards to surface roughness SHC group showed no significant differences compared to distilled water (DW) and dry samples (P˂0.180 and P˂0.176 respectively). While HPO groups displayed higher surface roughness with significant differences (P˂0.000, SHC P˂0.003, and CHX P˂0.018). The CHX groups showed also higher surface roughness compared to DW, Dry and SHC samples (P˂0.000), as well as with HPO P˂0.018). The microscope graphs of SHC showed the no clear effect on surface. The HPO and CHX showed a clear effect on surface roughness. 5% SHC recommended to be used as disinfectant agent rather than HPO and CHX agents.