Chemical Science & Engineering Research
Title
Evaluation of Antimicrobial Activity, Brine Shrimp Assay and Anticancer Efficacy (MCF 7 Cell Lines) of Cinnamomum Zeylanicum Bark Extract Mediated AgNPs
Authors
Supraja N.,*a Rajasekhar K.K.,b Kishore B.c and Bhavitha J.b
aAcharya N G Ranga Agricultural University, Institute of Frontier Technology, R.A.R.S, Nanotechnology laboratory, Tirupathi-517507, India.
bDepartment of Pharmaceutical Analysis, Sri Padmavathi School of Pharmacy, Tiruchanoor, Tirupati-517503, India.
cDepartment of Pharmaceutics, Sri Padmavathi School of Pharmacy, Tiruchanoor, Tirupati-517503, India.
*Corresponding author E-mail address: krishna.supraja@gmail.com (Supraja N)
Article History
Publication details: Received: 03rd September 2021; Revised: 19th October 2021; Accepted: 19th October 2021; Published: 02nd November 2021
Cite this article
Supraja N.; Rajasekhar K.K.; Kishore B.; Bhavitha J. Evaluation of Antimicrobial Activity, Brine Shrimp Assay and Anticancer Efficacy (MCF 7 Cell Lines) of Cinnamomum Zeylanicum Bark Extract Mediated AgNPs. Chem. Sci. Eng. Res., 2021, 3(8), 22-29.
Abstract
Cinnamomum zeylanicum exhibit potential antimicrobial, antioxidant and antifungal properties. Furthermore, cytotoxic and apoptotic activities of several constituents are been recent addition to its biological properties. In the present study, biosynthesized silver nanoparticles using bark extract of Cinamomum zeylanicum were evaluated for their antibacterial and antifungal activities against scale forming bacteria and fungi in PVC pipelines. The synthesis of nanoparticles was characterized by UV-visible spectroscopy carried out to quantify the formation of silver nanoparticles for localized surface Plasmon resonance (LSPR) at 420nm. Fourier transform infrared spectroscopic (FT-IR) analysis revealed that primary, secondary amine groups in combination with the proteins present in the bark extract are responsible for the reduction and stabilization of the AgNPs. X-ray diffraction (XRD) micrograph indicated the face centered cubic (FCC) structure of the formed AgNPs and morphological studies including size (average size 50nm) were carried out using transmission electron microscopy (TEM). The hydrodynamic diameter (HDD) (60.6nm) and zeta potential (ZP) (10.4mV) were measured using the dynamic light scattering technique (DLS). The antimicrobial activity as prepared silver nanoparticles was tested against Fungal sp, Gram negative and Gram positive bacteria through disc diffusion method. Further, the cytotoxic effects of synthesized AgNPs were evaluated against Human Breast Cancer (MCF 7) cell line.
Keywords
AgNPs; Antimicrobial activity; Bark extract of Cinamomum zeylanicum, Brine shrimp assay, MCF7 cell lines
