Articles - Chemical Science & Engineering Research

Abstract

The biological synthesis of AgNPs is cost effective and eco-friendly to that of conventional method of nanoparticles synthesis. In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated to evaluate antibacterial and antifungal activities through biosynthesis of AgNPs using bark extract of Boswellia ovalifoliolata, Stable AgNPs were formed by treatment of aqueous solution of AgNO3 (1mM). The formation of BAgNPs was confirmed by UV– analysis and recorded the localized surface plasmon resonance (LSPR) at 430nm. FT-IR analysis revealed that primary and secondary amine groups in combination with the proteins present in the bark extract are responsible for the reduction and stabilization of the BAgNPs. The morphology and crystalline phase of the nanocrystals were determined by TEM and XRD. The hydrodynamic diameter (96.8nm) and a negative zeta potential (-14.6mV) were measured using the dynamic light scattering technique. The antimicrobial activity of AgNPs was evaluated (in vitro) against fungi, bacteria using disc diffusion method which were isolated from the scales formed in drinking water PVC pipelines. MTT assay studied by HepG2 cell lines showed significant cytotoxic activity with IC50 value. In, the present study nanoparticles shown very good Antibiofilm activity towards fungi and bacteria isolated from PVC pipeline biofilm and these types of nanoparticles will show major challenge is designing particles that have long circulation times and low toxicity. Herein, we suggest green synthesis of AgNPs with potent antibacterial, antifungal, and anti-scaling activities with feasible biomedical and industrial applications and the scope for further development of anticancer drugs.