Title
Detection of Plant Growth Promoting Traits in Pseudomonas lurida AH1 Isolated from Rhizosphere of Aconitum Heterophyllum wall. ex royle
Authors
Neha Gautam,*a Pankaj Sharma,b Jai Chand Ranac and Mohar Singhd
aDepartment of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar, University of Horticulture and Forestry, Nauni, Solan, –173 230, H.P., India.
bHimachal Pradesh State Council for Educational Research and Training, Solan, H.P., India.
cDivision of Germ plasm evaluation, National Bureau of Plant Genetic Resource, New Delhi, India.
dNational Bureau of Plant Genetic Resource, Regional Station, Phagli, Shimla, H.P., India.
*Corresponding author E-mail address: neha_mbg@yahoo.com (Neha Gautam)
Article History
Publication details: Received: 21st September 2021; Revised: 26th October 2021; Accepted: 26th October 2021; Published: 19th November 2021
Cite this article
Neha G.; Pankaj S.; Rana J.C.; Mohar S. Detection of Plant Growth Promoting Traits in Pseudomonas lurida AH1 Isolated from Rhizosphere of Aconitum Heterophyllum wall. ex royle. Green Rep., 2021, 2(6), 26-31.
Abstract
Psychrotrophic microbial inoculants are vital for temperate agriculture and horticulture, as major part of the crop season is characterized by cold. In the present study, an attempt has been made to isolate psychrotrophic Pseudomonas lurida from rhizosphere of Himalayan medicinal herb Aconitum heterophyllum Wall ex. Royale. The study suggests the possibility to explore potential microflora associated with rarely explored plant wealth of Himalaya. Psychrotrophic Pseudomonas lurida AH1 isolated by using traditional microbiological techniques from rhizosphere of Aconitum heterophyllum wall. Ex royle- a medicinal plant grown on high altitude of Northwest Himalaya. Bacterial isolate Pseudomonas lurida AH1 was identified by 16SrRNA gene sequence and has been registered in NCBI under the accession number MG74660. The strain was characterized for plant growth promoting traits viz., Phosphate Solubilization, Ammonia Production, HCN production, Protease production. Antagonistic activity of isolated strain against five soil-borne plant pathogens was also studied by using a dual culture in vitro assay. The present study proposes the use of Pseudomonas lurida AH1 as a potential plant growth promoting rhizobacteria in the context of cold agro-ecosystems. Pseudomonas lurida AH1 is capable to produce siderophore, HCN, ammonia, protease and has potential to solubilize phosphates. Also in the present investigation, Pseudomonas lurida AH1 was tested against deadly plant pathogens viz., Alternaria solani, Botrytis cinera, Fusarium oxysporum, Rhizoctonia solani and Rosellina sp.
Keywords
Biofertilizers; Psychrotrophs; Pseudomonas; PGPR; Proteolysis; Siderophores
